중합효소 연쇄반응법을 이용한 Fusobacterium nucleatum ATCC 25586의 동정
Identification of Fusobacterium nucleatum ATCC 25586 using polymerase chain reaction
소속 상세정보
1국승호/1Seung Ho Gug
1김동기/2성진효/3국중기/1Dong Kie Kim/2Jin HYo Seong/3Joong Ki Kook
KMID : 0355419990230040329
Abstract
-Abstract-
The purpose of the present study was to develop DNA probe and polymerase chain
reaction (PCR) primer for rapid and accurate detection and identification of
Fusobacterium nuleatum ATCC 25586. This study procedure includes (1) whole-genomic
DNA extraction of Fusobacterium nucleatum ATCC 25586, (2) construction of the
genomic DNA library, (3) screening of restraction fragment of genomic DNA, (4)
identification of species-specific DNA probe by Southern blot hybridization, (5)
determination of nucleotide sequences of species-specific DNA probe, (6) design of PCR
primer, (7) PCR. Three restriction fragments of Fusobacterium nucleatum ATCC 25586
genomic DNA digested with the endonuclease XbaⅠ were obtained. The probe F25 was
shown to be hybridized with Fusobacterium nucleatum ATCC 25586 strongly. The
nucleotide sequences of probe F25 (830 base pairs) was obtained by dideoxy-chain
termination method. From that results, the specific primers (GSH1 ＆ GSH2) to
Fusobacterium nucleatum ATCC 25586 were designed. It has been found that primer
GSH1 and GSH2 could detect Fusobacterium numleatum ATCC 25586 using PCR.
These data indicated that these DNA probe and PCR primers could be used to detect
and identify the Fusobacterium nucleatum ATCC 25586.
키워드
치주병인균; 핵산염기서열; DNA probe; F. nucleatum; hybridization; PCR; DNA probe; F. nucleatum; hybridization; Nucleotide sequences; Periodontal pathogens; PCR;
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